First molecular identification of Sarcocystis hirsuta in Iranian beef: A case report
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Abstract:
Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S. hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The 18SrRNA gene as a specific target gene was used for molecular detection of Sarcocystis spp, then Restriction Fragment Length Polymorphysm (RFLP) analysis identified the speciesusing Rsa and Bfa. Results showed that our designed molecular method could identify S. hirsuta in beef sample. Conclusion: Based on our knowledge, this study indicates the first report of molecular identification of S. hirsuta in Iran.
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First molecular identification of Sarcocystis hirsuta in Iranian beef: A case report
Introduction: The main agents of sarcocystosis in cattle as an intermediate host include S. cruzi, S. hominis and S. hirsuta. A sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. Case report: After collection of beef sample from Yazd slaughterhouse, DNA extraction was done with salting out method. The 18SrRNA gene as a specific target gene...
full textfirst molecular identification of sarcocystis hirsuta in iranian beef: a case report
introduction: the main agents of sarcocystosis in cattle as an intermediate host include s. cruzi, s. hominis and s. hirsuta. a sensitive and specific tool such as molecular-based techniques would be necessary to identify the species. case report: after collection of beef sample from yazd slaughterhouse, dna extraction was done with salting out method. the 18srrna gene as a specific target gene...
full textFirst detection of Sarcocystis hirsuta from cattle in Iran
Although the high infection rate of microscopic sarcocyst occurs in cattle, to the best of our knowledge,there is no report about the identification of macroscopic sarcocyst in Iran. In this work, Sarcosystis hirsutawas first isolated from slaughtered cattle. In a 2-year-old slaughtered cattle, morphological examinationrevealed 3-5 mm in length cysts distributed in different striated muscles, p...
full textSarcocystis cruzi: First molecular identification from cattle in Iran
Sarcocystis is a genus of cyst-forming parasites infecting both animals and human. This study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of Sarcocystis cruzi from the samples. The samples were obtained from commercial source in Babol, Northern Iran. Five grams of calf muscle was cut into 2-3 pieces in 30 ml ...
full textsarcocystis cruzi: first molecular identification from cattle in iran
sarcocystis is a genus of cyst-forming parasites infecting both animals and human. this study aimed to isolate coccidian tissue cysts from muscle of infected animals by a simple method in addition to molecular identification of sarcocystis cruzi from the samples. the samples were obtained from commercial source in babol, northern iran. five grams of calf muscle was cut into 2-3 pieces in 30 ml ...
full textfirst detection of sarcocystis hirsuta from cattle in iran
although the high infection rate of microscopic sarcocyst occurs in cattle, to the best of our knowledge,there is no report about the identification of macroscopic sarcocyst in iran. in this work, sarcosystis hirsutawas first isolated from slaughtered cattle. in a 2-year-old slaughtered cattle, morphological examinationrevealed 3-5 mm in length cysts distributed in different striated muscles, p...
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Journal title
volume 1 issue 1
pages 32- 34
publication date 2014-03
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